The department had said in a statement on Monday that the Israeli military has assured Canada more than 400 of its citizens would be able to cross "in the coming days."
More Canadians could soon be leaving Gaza after their names appeared in the latest published document by Gaza's General Authority of Crossings and Borders.
The Canadian government confirmed that 75 people connected to Canada left the Palestinian territory via its border with Egypt on Tuesday.
Global Affairs Canada said that Canada is unable to determine when or how many people can cross each day, and the situation is fluid and unpredictable.
It said Canadian officials were on the Egyptian side of the border to welcome those who are leaving and facilitate their transportation to Cairo.
The department had said in a statement on Monday that the Israeli military has assured Canada more than 400 of its citizens would be able to cross "in the coming days."
The breakthrough on Tuesday came after Canada had told citizens trapped in Gaza they could be allowed out over the weekend, but attacks by Israel closed the Rafah crossing the only way out for foreign nationals until Monday.